Puguh Indrasetiawan

27 Desember 2011

Master Student, Biomedical Science, UGM

Research title:

Alpha terpineol activity as a anticancer candidate on T47-D cell line: Cytotoxycity, proliferation hydrance, and apoptosis stimulation

Outline:

Breast cancer is the disease that spreads all over the world. In Indonesia, patients who have breast cancer are treated with chemotherapy, radiation, surgery and immunotherapy (and combination of them), but those treatments cannot be 100% effective against cancer. Chemotherapy using drugs such as doxorubicin and tamoxifen has lots of side effect, and now we have a new strategy to combat cancer: using natural compounds from plants that have very few side effects, one of them is alpha-terpineol, a volatile oil obtained from Pinus merkusii, the native plant of Indonesia.

            This research is focused on how alpha-terpineol, volatile oil from Pinus merkusii, can affect breast cancer cell line. The cell line used is T47D, which will resemble the breast cancer in human. To determine wheter the activity of alpha-terpineol extracted fromPinus merkusii is good or not, it will be compared to alpha-terpineol that is sold commercially. And to determine the specifity of alpha-terpineol extracted from Pinus merkusii, the normal cell line (Vero) is used. Vero

            The parameters observed are the apoptotic profile, cytotoxicity and the cell cycle arrest profile of T47D cell line that is treated with alpha-terpineol extracted from Pinus merkusii, and comparing them (the three parameters) to control and normal cell line group. It is hoped that alpha-terpineol will selectively combat T47D cell line and having non-toxic activity against the

It is expected to have good result, so we can continue it to the higher level, animal model. And hopefully alpha-terpineol can continue its journey to the clinical trial through a lot of experiements.