Rasuane Noor

27 Desember 2011

Master Student, Biomedical Science, UGM

Research title:

Cytotoxic activity Α-terpineol As a candidate anticancer On Cell Line Helah And Its Effect on Apoptosis As well as the cell cycle

Outline:

            Cancer is the leading cause of death and disease in addition to cardiovascular disease because of infection. In Indonesia, cancer is the leading cause of death number seven (5.7%) after stroke, tuberculosis, hypertension, injury, perinatal, and DM. The number of cancer patients continues to increase with the prevalence 4.3 per 1000 population. Most cancer cases in Indonesia are cervical cancer, breast cancer, skin and lung (MOH, 2009).

 Cervical cancer is the highest cancer suffered by women in the world today, in America in 2004, approximately 10.500 new cases, and recorded 3900 cases of death from cancer. In Indonesia, the incidence of cervical cancer was 16 per 100,000 women. Data of hospitalized cancer patients in the hospital from 2004 to 2008, cervical cancer ranks second, after breast cancer (MOH, 2009).

The high incidence and mortality of cancer today is due to relatively low treatment success rate. Although various methods of healing done include surgery, radiation, immunotherapy, and chemotherapy, of each method that has been conducted have weaknesses of each so that the success rate still low (Hoffman, 1999).

          Various strategies have been implemented in the discovery of anticancer, one of them is to isolate the active compounds from natural materials. Compounds that inhibit the growth of cancer cells specifically and very important role as anticancer. One compound that is currently widely studied is the class of terpenoids, because of the anticancer effects of terpenoids against cancer cells (Zhou, 2005).

            Monoterpene obtained from various plants such as eucalyptus (Melaleuca leucadendra), pine (Pinus sp.) and lime (Citrus aurantifolia) is terpineol (Anonymous, 1989). Terpineol can be produced from synthesis negligent pinene from turpentine content of the extract taken from the sap of pine trees (Pinus) (Aquirre, et al. 2005). α-terpineol were reported as natural compounds as anticancer potential, which proved to inhibit growth and induce tumor cell death through a mechanism involving inhibition of NFkB activity (Hassan et al. 2010).

            Α-terpineol compounds used have never studied its activity in HeLa cervical cancer cells. This research was conducted to assess the anticancer effects of terpineol on HeLa cervical cancer cells. In this study also wanted to study whether cell death caused by cervical cancer HeLa apoptotis process and its effects on cell cycle.